Oligobind Thrombin: a novel oligonucleotide-based enzyme capture assay for measurement of free thrombin in human plasma

The Oligobind Thrombin Assay (product no. ADG844) is performed in 96-well microtiter plate format. Immobilized HD1-22, a bivalent and high-affinity thrombin-binding DNA aptamer, is used to capture thrombin from plasma samples. Since HD1-22 binds thrombin through both anionic exosites the active centre is not occupied. This allows quantification of aptamer-bound thrombin through hydrolysis rates of a fluorogenic peptide substrate. To prevent inactivation of thrombin prior to analysis, a protease inhibitor that reversibly blocks the active center of thrombin is added to special blood collection tubes that are needed for blood drawing. Total assay time is less than four hours, allowing the utilisation of the Oligobind Thrombin in daily routine practice.

Taken together, the Oligobind Thrombin assay is a powerful diagnostic tool that allows sensitive measurement of free thrombin in human plasma.

The assay is now available from American Diagnostica GmbH